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KMID : 1189220100370020125
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Korean Journal of Reproductive Medicine
2010 Volume.37 No. 2 p.125 ~ p.134
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Effect of Human Hydrosalpingeal Fluid on the Development of Mouse Embryo
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Park Joon-Cheol
Kim Jeong-A
Kim Dong-Ja
Bae Jin-Gon
Kim Jong-In
Rhee Jeong-Ho
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Abstract
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Objective: The aim of this study was to measure the concentrations of cytokines contained in the hydrosalpingeal fluid and to evaluate the effect on the mouse embryo development with the different cytokine concentration.
Methods: The hydrosalpingeal fluids (HSF) were collected during the surgery for hydrosalpinx which was confirmed by hysterosalphingogram. The cytokines in HSF including interleukin (IL)-1α, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, TNF-α, Interferon-γ (IFN-γ), vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), monocyte chemotactic protein-1 (MCP-1) were measured by ELISA method. HSF were added up to culture media with 5%, 10%, and 30% concentrations. The blastulation rates were compared.
Results: IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, TNF-α, IFN-γ, VEGF, EGF, and MCP-1 were detected, but the concentrations were different from each sample. IL-6 and IL-10 were increased in HSF-1 group, and IFN-γ, MCP-1, VEGF were increased in HSF-2 compared with normal serum range. The Th1/Th2 ratio of HSF-2 (IFN-γ:IL10) was highly elevated to 61.64, compared with that of HSF-1 (3.69). The blastulation rate was significantly decreased in HSF-2 group (27.7%) compared those of the HSF-1 group (74%) and control group (76.7%). It showed the trend that the blastulation rate was decreased depending on the concentration HSF of culture media in HSF-2 group, but it was not statistically significant.
Conclusion: The composition and concentration of cytokines in each HSF were different, and increased proinflammatory cytokines in HSF might be associated with poor embryonic development. Further study will be needed about the effect of each cytokines in HSF.
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KEYWORD
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Hydrosalpingeal fluid, Cytokine, Mouse embryo, Blastulation rate
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